Bindesh Shrestha - George Washington University

Last Updated: February 04, 2009

Bindesh Shrestha
Research Assistant
Department of Chemistry
George Washington University

Research Director

Professor Akos Vertes
http://www.gwu.edu/~vertes/
http://www.gwu.edu/~ipta/

Research Interest

My research interest includes the fundamentals and applications of soft-ionization mass spectrometry to solve biological problems. I am currently interested in infrared-laser based ambient soft-ionization techniques, such as Laser ablation electrospray ionization (LAESI)and atmospheric pressure (AP) infrared (IR) matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, to perform direct and rapid in situ analysis of metabolites and lipids in tissue.

Contact Information

Email: bindesh (at) gwmail.gwu.edu
Phone: 994 6344 (Area code 202)

Office/Lab

Vertes Research Laboratory 407, Corcoran Hall
725 21st Street, NW Washington, DC 20052

Conference Proceedings*

B. Shrestha, P. Nemes, and A. Vertes, “Laser ablation and analysis of a single cell”, 10th International Conference on Laser Ablation, Singapore, November 22-27, 2009.| Abstract |

A. Vertes, P. Nemes, and B. Shrestha,"Laser Ablation Electrospray Ionization (LAESI) Mass Spectrometry for Direct Analysis and Imaging of Cells and Tissues", 36th The Federation of Analytical Chemistry and Spectroscopy Societies (FACSS) Conference, Lake Buena Vista, FL, October 18-22, 2009. | Abstract |

B. Shrestha and A. Vertes, "Metabolic profiling of a single live cells by laser ablation electrospray ionization mass spectrometry",Proceedings of the 57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA, May 31 - June 4, 2009; DVD-ROM.
| Abstract | Poster |

C. H. Stephens, B. Shrestha, P. M. Whitmore, M. E. Bier, and A. Vertes, "Optimization of three atmospheric pressure mass spectrometry (AP-MS) techniques to observe oligosaccharide degradation products in naturally and artificially aged paper",Proceedings of the 57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA, May 31 - June 4, 2009; DVD-ROM. | Abstract | Poster |

P. Nemes, B. Shrestha, and A. Vertes, "Mid-infrared laser ablation electrospray ionization (LAESI) for spatial imaging of tissue metabolites with mass spectrometry", Desorption Induced by Electronic Transition (DIET) XII, Pine Mountain, GA, April 19-23, 2009.
| Abstract |

P. Nemes, B. Shrestha, and A. Vertes, "Direct tissue and single cell metabolomics by LAESI mass spectrometry", Pittcon 2009, 2009. | Abstract |

C. H. Stephens, B. Shrestha, P. M. Whitmore, H. R. Morris, M. E. Bier, and A. Vertes, "Mass spectroscopic analysis of soluble cellulose oligomers in naturally and artificially aged paper", 237th ACS National Meeting, Salt Lake City, UT, March 22-26, 2009. | Abstract | Poster |

B. Shrestha,P. Nemes, P. Sripadi, J. Nazarian, Y. Hathout, S. Trikha, A. Jeremic, E. P. Hoffman, S. A. Moody and A. Vertes, “Lipid analysis from amphibian eggs, cell pellets and tissues by reactive LAESI mass spectrometry”, Proceedings of the 21st ASMS Sanibel Conference "Lipidomics and Lipids in Mass Spectrometry", St. Pete's , FL, January 23-26, 2009. | Abstract | Poster |

A. Vertes, P. Nemes, A. A. Barton, B. Shrestha, and Y. Li, “In vivo analysis and imaging of biological tissue with infrared laser ablation mass spectrometry”, 236th American Chemical Society National Meeting, Philadelphia, PA, August 17-21, 2008. | Abstract |

B. Shrestha, P. Nemes, J. Nazarian, E. P. Hoffman, and A. Vertes, “Direct analysis of lipids and other metabolites in mouse brain tissue with infrared laser ablation and mass spectrometry”, Proceedings of the 56th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO, June 1-5, 2008; DVD-ROM.
| Abstract | Poster |

B. Shrestha, Y. Li, and A. Vertes, “Rapid analysis of pharmaceuticals and their metabolites with atmospheric pressure infrared MALDI mass spectrometry”, Proceedings of the 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN, June 3-7, 2007; DVD-ROM. | Abstract | Poster |

Y. Li, B. Shrestha, and A. Vertes, “Atmospheric pressure infrared MALDI mass spectrometry: imaging and in vivo studies of metabolites in plants”, Proceedings of the 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN, June 3- 7, 2007; DVD-ROM. | Abstract |

B. Shrestha, Y. Li, and A. Vertes, “Direct analysis of drugs and their metabolites by infrared atmospheric pressure matrix-assisted laser desorption ionization mass spectrometry”, 33rd The Federation of Analytical Chemistry and Spectroscopy Societies (FACSS) Conference, Lake Buena Vista, FL, September 24-28, 2006. | Abstract | Poster |

Y. Li, B. Shrestha, and A. Vertes, “Atmospheric imaging at atmospheric pressure by MALDI mass spectrometry”, Proceedings of the 54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA, May 28-June 1, 2006, DVD-ROM. | Abstract | Poster |

J. Nazarian, B. Shrestha, Y. Hathout, A. Vertes, and E. P. Hoffman, “Proteomic analyses of Torpedo californica electroplaque as a model for the neuromuscular junction”, Proceedings of the 54th American Society for Mass Spectrometry (ASMS) Conference on Mass Spectrometry and Allied Topics, Seattle, WA, May 28-June 1, 2006, DVD-ROM. | Poster |

* The presenting author(s) are underlined.

About me

I am a postdoctoral research scientist at the Chemistry Department of the George Washington University (GWU), Washington DC. My research director is Professor Akos Vertes.

I received undergraduate degree from Moravian College , Bethlehem , PA with double major in Chemistry and Economics. I received PhD from the George Washington University.

News & highlights

October 2009

Analytical Chemistry reports on Exploring cell insides by explosion on analysis possibility of the contents of a single live cell that is functioning normally.

October 2009

Our article on analysis of single cell was featured on the cover page of the Analytical Chemistry (October 20, 2009 / Volume 81 / Issue 20).

March 2008

Biophotonics featured a report on metabolite analysis by AP IR-MALDI - Deeper Into The Plant Kingdom .

January 2008

Our article on atmospheric pressure analysis of plant metabolomics was featured on the cover page of the Analytical Chemistry (January 15, 2008 / Volume 80 / Issue 2).

Peer Reviewed Publications

B. Shrestha and A. Vertes, “In Situ Metabolic Profiling of Single Cells by Laser Ablation Electrospray Ionization Mass Spectrometry,” Analytical Chemistry, 2009, 81, 8265-8271. Supporting information. (Accelerated Articles) (DOI: 10.1021/ac9021314)

B. Shrestha, Y. Li, and A. Vertes, “Rapid analysis of pharmaceuticals and excreted xenobiotic and endogenous metabolites with atmospheric pressure infrared MALDI mass spectrometry”, Metabolomics, 2008, 4, 297-311.(DOI: 10.1007/s11306-008-0120-8)

A. Vertes, P. Nemes, B. Shrestha, A. A. Barton, Z. Chen, and Y. Li,”Molecular Imaging by Mid-IR Laser Ablation Mass Spectrometry,” Applied Physics A, 2008, 93, 885-891. (DOI: 10.1007/s00339-008-4750-5)

Y. Li, B. Shrestha, and A. Vertes, “Atmospheric Pressure Infrared MALDI Imaging Mass Spectrometry for Plant Metabolomics,” Analytical Chemistry, 2008, 80, 407-420. (DOI: 10.1021/ac701703f)

Y. Li, B. Shrestha, and A. Vertes, "Atmospheric Pressure Molecular Imaging by Infrared MALDI Mass Spectrometry," Analytical Chemistry,2007, 79, 523-532. (DOI: 10.1021/ac061577n)

Any comments, email me at bindesh(AT)gwmail.gwu.edu